The effects of resveratrol treatment on caveolin-3 expression and Na+/K+ ATPase activity in rats with isoproterenol-induced myocardial injury

OBJECTIVE: The present study aims to investigate the therapeutic effects of resveratrol (RES) on isoproterenol (ISO) induced myocardial injury rat model.
METHODS: Catecholamine-induced heart damage was induced by ISO treatment for 30 days. The rats were divided into four groups as follows: the control group received saline, the ISO group received 5.0 mg/kg ISO, the RES group received 10 mg/kg RES, and the ISO-RES group received 10 mg/kg RES and 5 mg/kg ISO treatments for 30 days. Following echocardiographic measurements and body weight recorded, the rats were decapitated. Plasma and cardiac tissue samples obtained by decapitation were analyzed using biochemical, histopathological, molecular and immunohistochemical methods.
RESULTS: In the ISO group, Na+/K+ ATPase activity and ATP content, GSH, and caveolin-3 levels were low. LDH, CK and lysosomal enzyme activities, MDA level, and MPO activity were found to be high. It was determined that GSH and MDA levels and MPO, Na+/K+ ATPase activity, ATP content caveolin-3 levels changes that arose from ISO treatment were suppressed by RES treatment.
CONCLUSION: RES treatment has ameliorated all the functional and biochemical parameters. The results obtained in this study suggest that RES is a promising supplement against catecholamine exposure as it improves antioxidant defense mechanisms in the heart. In the light of above-mentioned data, RES can be assumed as a promising agent in ameliorating the oxidative injury of the myocardium.

Resveratrol tedavisinin izoproterenol ile indüklenen miyokard hasarý sýçan modelinde caveolin-3 ekspresyonu ve Na + / K + ATPaz aktivitesi üzerine etkisi

AMAÇ: Bu çalýþmanýn amacý, resveratrolün (RES) izoproterenol (ISO) kaynaklý miyokard hasarý sýçan modelinde terapötik etkisini araþtýrmaktýr.
YÖNTEMLER: Katekolamin kaynaklý kalp hasarý, 30 gün boyunca ISO tedavisi ile indüklenmiþtir. Sýçanlar 4 gruba ayrýldý: kontrol grubu salin, ISO grubu 5.0 mg / kg ISO, RES grubu 10 mg / kg RES ve ISO-RES grubu 10 mg / kg RES ve 5 mg / kg ISO tedavisini 30 gün boyunca almýþtýr. Ekokardiyografik ölçümler ve vücut aðýrlýðý kaydedildikten sonra, sýçanlar dekapite edilmiþtir. Dekapitasyon ile elde edilen plazma ve kalp dokusu örnekleri biyokimyasal, histopatolojik, moleküler ve immünohistokimyasal yöntemler kullanýlarak analiz edilmiþtir.
BULGULAR: ISO grubunda Na + / K + ATPaz aktivitesi ve ATP içeriði, GSH ve caveolin-3 seviyeleri düþük; LDH, CK ve lizozomal enzim aktiviteleri, MDA düzeyi ve MPO aktivitesi yüksek olduðu belirlenmiþtir. ISO tedavisinin neden olduðu GSH, MDA, MPO, Na + / K + ATPase aktivitesi, ATP içeriði caveolin-3 düzey deðiþikliklerinin RES tedavisi ile baskýlandýðý tespit edilmiþtir.
SONUÇLAR: RES tedavisi tüm fonksiyonel ve biyokimyasal parametreleri iyileþtirmiþtir. Bu sonuçlar, RES’in kalpteki antioksidan savunma mekanizmalarýný geliþtirdiði için kalekolamin maruziyetine karþý umut verici bir destek olduðunu göstermektedir. Yukarýda belirtilen veriler ýþýðýnda, RES, miyokardýn oksidatif hasarýný iyileþtirmede ümit verici bir ajan olarak kabul edilebilir. (NCI-2019-0191.R1)