North Clin Istanb. Ahead of Print: NCI-76735 | DOI: 10.14744/nci.2021.76735

The Clinical Importance of Leukocyte Associated Immunoglobulin like Receptor 1 in Chronic Lymphocytic Leukemia Patients

Hacer Berna Afacan Ozturk¹, Ahmet Kursad Gunes², Murat Albayrak¹, Umit Yavuz Malkan¹, Mesude Falay³, Senem Maral¹, Gulsum Ozet^2
1University of Health Sciences, Diskapi Yildirim Beyazit Training and Research Hospital, Department of Hematology, Ankara, TURKEY
²University of Health Sciences, Ankara City Hospital, Department of Hematology, Ankara, TURKEY
³Düzen Laboratory Groups, Ankara, TURKEY

Objective: Leukocyte-associated immunoglobulin-like receptor 1 (LAIR1) also named as CD305, is a transmembrane glycoprotein that is expressed various immune cells. The expression of LAIR1 varies during the differentiation of B cells and recently LAIR1 expression was confirmed in CLL. The exact role and prognostic importance of the expression of LAIR1 is not yet clarified in CLL patients.
Methods: We have aimed to clarify the prognostic importance of the LAIR1 expression in CLL patients by analyzing the LAIR1 expressions of sixty two de novo CLL patients using the flow cytometric tests.
Results: There was a statistically significant difference in the serum beta-2 microglobulin levels of LAIR positive patients compared to LAIR negative patients (t=2,162; p=0,040). Serum beta-2 microglobulin levels were found to be higher in patients who have LAIR1 expression lower than 30, compared to patients who have LAIR1 expression 30 or more (p<0.05). According to CLL-IPI prognostic system, the higher levels of serum beta-2 microglobulin levels lead to worse prognosis of CLL; which indirectly confirms the relation detected in our study between the low LAIR1 levels and high serum beta-2 microglobulin levels showing prognostic course in CLL patients.
Conclusion: In conclusion, the low levels of LAIR1 expression in CLL patients is related with poor prognosis. These results may lead to a potential treatment target in CLL patients in the era of novel targeted drugs. There is a need for future prospective studies that focuses on LAIR1 and its role within the other genetic and prognostic factors.

Keywords: Leukocyte Associated Immunoglobulin like Receptor 1 (CD305), Chronic Lymphocytic Leukemia, Beta-2 microglobulin, Prognosis, Flow cytometry

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Kronik Lenfositik Lösemi hastalarýnda Lökosit Ýliþkili Ýmmunoglobulin Benzeri Reseptör 1'in klinik önemi

Hacer Berna Afacan Ozturk¹, Ahmet Kursad Gunes², Murat Albayrak¹, Umit Yavuz Malkan¹, Mesude Falay³, Senem Maral¹, Gulsum Ozet^2
1Saðlýk Bilimleri Üniversitesi, Dýþkapý Yýldýrým Beyazýt Eðitim ve Araþtýrma Hastanesi, Hematoloji Kliniði, Ankara
²Saðlýk Bilimleri Üniversitesi, Ankara Þehir Hastanesi, Hematoloji Bölümü, Ankara
³Düzen Laboratuvar Gruplarý, Ankara

Giriþ: CD305 olarak da adlandýrýlan Lökosit Ýliþkili Ýmmunoglobulin Benzeri Reseptör 1 (LAIR1) pek çok immun kökenli hücre yüzeyinde ifade edilen bir transmembran proteindir. B hücre farklýlaþmasýnda deðiþen LAIR1 ifadesinin KLL’de de ifade edildiði, yakýn zamanda onaylandý. KLL hastalarýnda LAIR1 ifadesinin rolü ve prognostik önemi tam olarak netleþmemiþtir.
Yöntem: Akýþ sitometrik testler aracýlýðýyla 62 yeni taný KLL hastasýnýn LAIR1 ifadelerini analiz ederek, KLL hastalarýnda LAIR1 ifadesinin prognostik önemini netleþtirmeyi amaçladýk.
Bulgular: LAIR1 negatif hastalara kýyasla LAIR pozitif hastalarýn serum beta-2 mikroglobulin düzeylerinde istatistiksel olarak anlamlý fark vardý (t=2,162; p=0,040). LAIR1 ifadesi 30 ve üzerinde olan hastalar ile karþýlaþtýrýldýðýnda, LAIR1 ifadesi 30’dan düþük olanlarda serum beta-2 mikroglobulin düzeyi daha yüksek saptandý (p<0.05). KLL-IPI skorlama sistemine göre, yüksek serum beta-2 mikroglobulin düzeyi kötü prognozla ilerlemekte ve bu da çalýþmamýzda tespit edilen düþük LAIR1 düzeyleri ile KLL hastalarýnda prognostik seyir gösteren yüksek serum beta-2 mikroglobulin düzeyleri arasýndaki iliþkiyi dolaylý yoldan doðrulamaktadýr.
Sonuç: Sonuç olarak, KLL hastalarýnda LAIR1 ekspresyonunun düþük seviyeleri kötü prognoz ile iliþkilendirilmiþtir. Bu sonuçlara göre hedefe yönelik ilaçlar çaðýnda, KLL hastalarýnda potansiyel bir tedavi hedefine yol açabilir. LAIR1 ve rolünü, diðer genetik ve prognostik faktörler ile odaklayan ileriye dönük çalýþmalara ihtiyaç vardýr. (NCI-2021-1-16)

Anahtar Kelimeler: Lökosit Ýliþkili Ýmmunoglobulin Benzeri Reseptör 1 (CD305), Kronik Lenfositik Lösemi, Beta-2 mikroglobulin, Prognoz, Akýþ sitometri

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