Detection of human immunodeficiency virus (HIV) RNA in the sweat of HIV-infected patients

OBJECTIVE: Human immunodeficiency virus (HIV) infection is a significant health problem. Many studies reported that HIV was mainly transmitted through parenteral exposure, sexual activity, and body secretions, such as saliva and semen. Many people, including health-care providers and patient relatives, may easily contact with the sweat of HIV-infected patients. Although reference books assert that HIV does not transmit through sweat, to our knowledge, there is no systemic study which this statement is based upon. This study aims to investigate the potential of sweat to transmit HIV infection.
METHODS: This study included 31 treatment-naive HIV RNA-positive patients who were in the acute phase of the infection and 26 subjects with a negative HIV RNA test who had received antiviral treatment. A total of 57 sweat samples collected from intact skin areas were prospectively evaluated by polymerase chain reaction (PCR) for the presence of HIV RNA. HIV RNA levels in the blood samples were also noted.
RESULTS: HIV RNA was not detected by PCR in any sweat sample taken from HIV-infected HIV RNA-negative and -positive subjects.
CONCLUSION: The findings obtained in this study suggest that sweat by itself has no potential for transmitting HIV infection.

Human immunodeficiency virus (HIV) hastalarinin terinde HIV RNA saptanmasi

GÝRÝÞ ve AMAÇ: Human Immunodeficiency Virus (HIV) enfeksiyonu önemli bir saðlýk sorunudur. HIV'in esas olarak parenteral maruziyet, cinsel aktivite ve tükürük ve semen gibi vücut salgýlarý yoluyla iletildiði çeþitli çalýþmalarla bildirilmiþtir. Saðlýk çalýþanlarý ve hastalarýn yakýnlarý dahil olmak üzere birçok insan, HIV ile enfekte hastalarýn terleriyle kolaylýkla temas edebilir. Her ne kadar referans kitaplarý HIV'in terden geçmediðini ileri sürse de, bu ifadenin temel aldýðý sistemik bir çalýþma yoktur. Bu çalýþma, HIV hastalarýnýn terlerinin, enfeksiyon bulaþýcýlýðý açýsýndan potansiyelini araþtýrmayý amaçlamaktadýr.
YÖNTEM ve GEREÇLER: Bu çalýþmada hastalýðýn akut fazýnda bulunan 31 tedavi baþlanmamýþ HIV ile enfekte hasta, 26 anti-viral tedavi almýþ HIV RNA’sý negatif HIV ile enfekte hasta deðerlendirmeye alýnmýþtýr. Cilt bütünlüðü tam olan hastalardan prospektif olarak toplanan 57 serümen örneði HIV RNA varlýðý açýsýndan real-time polymerase chain reaction yöntemi ile incelendi. Ter örneði ile eþ zamanlý alýnan kan örnekleri incelenmiþtir.
BULGULAR: Ýncelenen 31 serum HIV RNA’sý pozitif ve 26 serum HIV RNA’sý negatif HIV ile enfekte hastanýn ter örneklerinde HIV RNA tespit edilememiþtir.
TARTIÞMA ve SONUÇ: Bu çalýþmanýn sonuçlarý, terinin kendi baþýna HIV enfeksiyonu geçirme potansiyeli olmadýðýný göstermektedir. (NCI-2018-0165.R2)