Examination of the expression levels of MACC1, Filamin A and FBXW7 genes in colorectal cancer patients

OBJECTIVE: Colorectal cancer (CRC) is the third most common type of cancer observed in cancer-related mortality because it has a high metastasis ratio. This study aims to investigate the expression levels of several genes, including metastasis-related colon cancer 1 (MACC1), Filamin A (FLNA), F-box/WD repeat-containing protein 7 (FBXW7), which has an important role in cell signaling, migration and adhesion through the remodeling of the cell skeleton.
METHODS: In this study, 21 patients with a precise diagnosis of CRC and 21 controls were included. Gene expressions were examined using the RT-PCR technique. To define the relationship of the genes with metastasis, blood samples were collected from all patients with colon/rectal cancer diagnosis without metastasis at six months before and after the medication with Xelox.
RESULTS: Our findings showed that no significant difference was observed in the pre-treatment values compared to the control group, whereas FLNA (p=0.001) expression was observed to be significantly increased following treatment with Xelox.
CONCLUSION: To our knowledge, our study is the first study to investigate the effects of Xelox treatment on the expression levels of MACC1, FBXW7 and FLNA genes in non-metastatic colorectal cancer patients in Turkey.

Kolorektal Kanser Hastlarýnda MACC1, Filamin A Ve FBXW7 Genlerinin Anlatým Düzeylerinin Incelenmesi

GÝRÝÞ ve AMAÇ: Kolorektal kanser, kansere baðlý ölümlerde yüksek metastaz oranýndan dolayý üçüncü sýrada yer almaktadýr. Bu çalýþmanýn amacý, hücre sinyali, göçü ve hücre iskeletinin yeniden þekillenmesinde görev alan metastaz iliþkili kolon kanser 1 (MACC1), filamin A (FLNA) ve F-box/WD tekrar içeren protein 7 (FBXW7) genlerinin anlatýmlarýnýn incelenmesidir.
YÖNTEM ve GEREÇLER: Kolorektal kanser tanýsý konulmuþ 21 hasta ve 21 kontrol çalýþmaya dahil edilmiþtir. Gen anlatýmlarý RT-PCR tekniði kullanýlarak saptanmýþtýr. Bu genlerin metastaz ile iliþkisinin saptanmasý amacýyla hastalardan Xelox tedavisi öncesinde ve altý ay sonrasýnda kan örnekleri toplanmýþtýr.
BULGULAR: Hastalarda tedavi öncesinde her üç genin anlatýmlarýnda bir farklýlýk bulunmamasýna karþýn, Xelox tedavisi sonrasýnda FLNA (p=0.001) gen anlatýmýnýn istatistiksel olarak arttýðý gözlemlenmiþtir.
TARTIÞMA ve SONUÇ: Bu çalýþma ülkemizde metastatik olmayan kolorektal kanseri hastalarýnda Xelox tedavisinin MACC1, FBXW7 ve FLNA gen anlatýmlarý üzerine etkisinin incelendiði ilk çalýþmalardandýr. (NCI-2018-0193.R2)